Abstract
The detection limits in two bioautographic assays have been determined for a series
of antifungal Compounds, including clinically used anti-mycotics, fungicidal agrochemicals,
and various classes of secondary plant metabolites. Target organisms were the filamentous
fungus Cladosporium cucumerinum and the yeast Candida albicans. For clinical agents and agrochemicals, the detection limits in the two assays re-flected
to a certain extent their known spectrum of activity. Most of the plant-derived Compounds
tested showed a positive response in both assays, but with detection limits varying
by a factor up to tenfold. For Screening purposes, it is thus advisable to use both
tests, as some activities would otherwise go undetected. The MIC values of these substances
were determined in Order to verify a possible correlation with the detection limit
in the bioautographic assays.
Key words
Cladosporium cucumerinum
-
Candida albicans
- antifungal - bioautography
