Abstract
Two diarylheptanoids, oregonin (1) and hirsutanone (2), were isolated by bioassay-guided fractionation of the methanol extracts of the
leaves of Alnus japonica Steud and their structures were elucidated from their spectroscopic data. Compounds
1 and 2 exhibited significant low-density lipoprotein (LDL)-antioxidant activities, e. g.,
thiobarbituric acid-reactive substances (TBARS) assay (1: IC50 = 3.2 μM, 2: IC50 = 1.5 μM), lag time (1 : 162 min, 2 : 230 min at 2.0 μM, control: 63 min), relative electrophoretic mobility (REM, inhibition
of 1 : 71 %, 2 : 75 % at 10 μM), apoB-100 fragmentation (inhibition of 1 : 27.3 %, 2 : 40.3 % at 10 μM) and macrophage-mediated LDL oxidation (inhibition of 1 : 82 %, 2 : 84 % at 1 μM).
Key words
Alnus japonica
- Betulaceae - low-density lipoprotein (LDL) - oregonin - hirsutanone
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Tae-Sook Jeong, Ph. D.
National Research Laboratory of Lipid Metabolism & Atherosclerosis
Korea Research Institute of Bioscience and Biotechnology
52 Oun-Dong
Yusong
Daejeon 305-333
Republic of Korea
Telefon: +82-42-860-4558
Fax: +82-42-861-2675
eMail: tsjeong@kribb.re.kr