Biomimetic Mechanism of Action of Fluoridated Toothpaste Containing Proprietary REFIX Technology on the Remineralization and Repair of Demineralized Dental Tissues: An In Vitro Study

Fabiano Vieira Vilhena; Sonia Mariza Luiz de Oliveira; Marcelo Henrick Maia Matochek; Pedro Luiz Santos Tomaz; Thales de Sá Oliveira; Paulo Henrique Perlatti D’Alpino

doi:10.1055/s-0040-1716781

European Journal of Dentistry, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(02): 236-241
DOI: 10.1055/s-0040-1716781

Original Article

Biomimetic Mechanism of Action of Fluoridated Toothpaste Containing Proprietary REFIX Technology on the Remineralization and Repair of Demineralized Dental Tissues: An In Vitro Study

Fabiano Vieira Vilhena

¹Trials Pesquisa e Desenvolvimento, Bauru, SP, Brasil

,

Sonia Mariza Luiz de Oliveira

²Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil

,

Marcelo Henrick Maia Matochek

²Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil

,

Pedro Luiz Santos Tomaz

²Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil

,

Thales de Sá Oliveira

²Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil

,

Paulo Henrique Perlatti D’Alpino

²Graduate Program in Biotechnology and Health Innovation, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil

³Graduate Program in Science Teaching and Health, Universidade Anhanguera de São Paulo (UNIAN-SP), São Paulo, Brazil

› Institutsangaben

Abstract

Objectives This in vitro study aimed to characterize the mineral content and surface and cross-sectional morphology of enamel and dentin tissues treated with a 1450 ppm fluoride-containing toothpaste with REFIX technology.

Materials and Methods Bovine enamel blocks (n = 5) were obtained (4 × 4 × 6 mm), demineralized (artificial caries lesion), and treated (pH cycling and brushing with the toothpaste). During the pH cycling, which lasted for 7 days (demineralization and remineralization took 6 and 18 hours, respectively), the enamel was brushed for 5 minutes using an electric toothbrush before being immersed in a remineralizing solution. The dentin blocks were acid-etched for 2 minutes (0.05 M citric acid, pH 1.8) to expose the dentinal tubules (n = 5). Morphological analysis of the dentin was performed immediately and after 7 days of brushing with the dentifrice, and compared with the control group. The specimens were then cross-sectioned. The surface and cross-sectional micromorphology were assessed using scanning electron microscopy (SEM). The elemental analyses (weight%) were determined with an energy-dispersive X-ray spectroscopy (EDS).

Results The toothpaste with REFIX technology remineralized and repaired the surface enamel effectively. The elemental analysis also demonstrated that treating the enamel with the toothpaste formed a silicon-enriched mineral layer on the enamel surface. Elemental analysis of the enamel cross-sections showed that the toothpaste induced a mineral change. The results were also consistent in the dentin, where the dentinal tubules were progressively occluded until there was complete occlusion after 7 days.

Conclusions We prove the biomimetic mechanism of action of fluoridated toothpaste containing proprietary REFIX technology for obtaining silicon-enriched, remineralized and repaired dental tissues.

Keywords

enamel - dentin - tooth remineralization - toothpaste - microscopy - electron - scanning

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