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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(03): 397-403
DOI: 10.1055/s-0040-1714453
Original Article

Anti-Inflammatory and Antibacterial Activity of the Chitosan/Chlorhexidine Gel Commercial Preparation for Postexodontia Treatment: An In Vitro Study

Rafael Torres-Rosas
1   Laboratorio de Inmunología, Centro de Estudios en Ciencias de la Salud y la Enfermedad, Facultad de Odontología, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México
,
Nayely Torres-Gómez
2   Centro Conjunto de Investigación en Química Sustentable, UAEM-UNAM, Facultad de Química, Universidad Autónoma del Estado de México, Toluca, México
,
Adriana Moreno-Rodríguez
3   Facultad de Ciencias Químicas, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México
,
René García-Contreras
4   Laboratorio de Investigación Interdisciplinaria, Área de Nanoestructuras y Biomateriales, Escuela Nacional de Estudios Superiores Unidad León; Universidad Nacional Autónoma de México, León, Guanajuato, México
,
Liliana Argueta-Figueroa
5   Cátedras-Conacyt - Facultad de Odontología, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, México
› Institutsangaben
Funding This study received its financial support from CONACyT (project number CB-2016–01–284495) assigned to RTR and DGAPA-PAPIIT: IA205518 assigned to RGC.
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Abstract

Objective The present study aimed to assess in vitro the antibacterial activity, cytotoxicity, and the expression of prostaglandin E2 (PGE2) of Bexident post topical gel (BP).

Materials and Methods The broth dilution test was performed to analyze the antimicrobial activity of BP against Staphylococcus aureus, Escherichia coli, and Streptococcus mutans. Minimal bactericidal concentrations (MBCs) and minimal inhibitory concentrations (MICs) were assessed. Cytotoxic activity was performed by the MTT (tetrazolium dye) method on human gingival fibroblast (HGF), human bone cells (HBC), and human pulp cells (HPC) (from primary cell culture) and HGF-1 from American Type Culture Collection. The expression of PGE2 produced by RAW 264.7 cells was determined by ELISA utilizing an Enzyme Immuno-Assay Kit.

Statistical Analysis Shapiro–Wilks normality test and Mann–Whitney U test were performed for all data.

Results The MBCs of BP for S. aureus, E. coli, and S. mutans were found at 25, 50, and 12.5%, respectively. The MICs for the same strains were found at 12.5, 25, and 3.125%. The CC50 of BP gel for HBC, HPC, and HGF, and HGF-1 were 12.5 ± 1.09, 0.37 ± 0.02, 0.35 ± 0.02, and 20.4 ± 0.02%, respectively. The levels of expression PGE2 produced by RAW 264.7 cells treated with IL-1β exhibit an inverse dose-dependent effect on the concentrations of BP gel used.

Conclusion Our results indicate that the BP gel has a great antibacterial effect, adequate biocompatibility, showing a decrease in the expression of PGE2 on cells with previously induced inflammation. Due to the above, its use as a healing agent after oral surgery seems to be adequate.

Keywords

chitosan - chlorhexidine - cytotoxicity - antibacterial properties - Bexident post


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Artikel online veröffentlicht:
20. Juli 2020

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