Human Platelets Contain Scinderin, a Ca2+-Dependent Actin Filament-Severing Protein

A Rodríguez Del Castillo; M L Vitale; L Tchakarov; J-M Trifaró

doi:10.1055/s-0038-1648420

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1992; 67(02): 248-251
DOI: 10.1055/s-0038-1648420

Original Articles

Schattauer GmbH Stuttgart

Human Platelets Contain Scinderin, a Ca²⁺-Dependent Actin Filament-Severing Protein

A Rodríguez Del Castillo

The Secretory Process Research Program, Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada

,

M L Vitale

The Secretory Process Research Program, Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada

,

L Tchakarov

The Secretory Process Research Program, Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada

,

J-M Trifaró

The Secretory Process Research Program, Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada

› Author Affiliations

Abstract

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Summary

A large body of biochemical and morphological evidence suggests that actin polymerizes in response to various stimuli which activate platelets. Previous work has shown the presence in platelets of gelsolin, a Ca²⁺-dependent regulator of actin filament length. This present work demonstrates that human platelets contain scinderin, another Ca²⁺-dependent actin filament-severing protein recently discovered in our laboratory. Extracts prepared from platelets were subjected to DNase-I-Sepharose 4B affinity chromatography. EGTA eluates from the affinity columns contained scinderin as demonstrated by mono and two-dimensional polyacrylamide gel electrophoresis and immunoblotting with scinderin antibodies. The concentration of scinderin in platelets was 75 fmol/mg total protein. This might represent 11% of the total actin filament-severing activity if both proteins are equally potent, on a molar basis, in severing actin filaments. Double staining immunocytochemical studies with antibodies against scinderin and rhodamine phalloidin, a probe for F-actin, also demonstrated the presence of scinderin in platelets. These findings suggest that scinderin may participate in the regulation of platelet actin networks.

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References

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