Insulin Stimulates the Synthesis of Plasminogen Activator Inhibitor 1 by the Human Hepatocellular Cell Line Hep G2

M C Alessi; I Juhan-Vague; T Kooistra; P J Declerck; D Collen

doi:10.1055/s-0038-1646997

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1988; 60(03): 491-494
DOI: 10.1055/s-0038-1646997

Original Article

Schattauer GmbH Stuttgart

Insulin Stimulates the Synthesis of Plasminogen Activator Inhibitor 1 by the Human Hepatocellular Cell Line Hep G2

Autoren

M C Alessi

¹The Laboratory of Hematology, CHU Timone, Marseille, France
I Juhan-Vague

¹The Laboratory of Hematology, CHU Timone, Marseille, France
T Kooistra

²The Gaubius Institute, TNO, Leiden, The Netherlands
P J Declerck

³The Center for Thrombosis and Vascular Research, Leuven, Belgium
D Collen

³The Center for Thrombosis and Vascular Research, Leuven, Belgium

Abstract

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Summary

Secretion of plasminogen activator inhibitor 1 (PAI-1) by cultures of human umbilical vein endothelial cells and human hepatocellular cell line Hep G2 was evaluated after insulin stimulation. The secretion of PAI-1 antigen and activity was measured in the conditioned medium and the cellular extracts after incubation of confluent cultures with 1% serum medium for 24 hours.

Insulin induced a dose dependent increase of the PAI-1 secretion by Hep G2 cell line. At 10^-8 M a two fold increase of PAI-1 antigen and activity were observed whereas a2 antiplasmin and fibrinogen were not significantly modified. No effect of insulin was observed on PAI-1 antigen and PAI activity production by human endothelial cells whereas endotoxin resulted in a two fold increase in PAI-1 secretion. In recent clinical studies we have demonstrated that the level of plasma insulin correlated with that of PAI-1. Thus we hypothesize that hepatocytes represent a physiological source of plasma PAI-1 which is modulated by plasma insulin level.

Keywords

Insulin - PAI-1 - Hepatocyte - Endothelial cell

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Referenzen

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