Antioxidant Activity and Induction of mRNA Expressions of Antioxidant Enzymes in HEK-293 Cells of Moringa oleifera Leaf Extract^[*]

Abstract

The leaves of Moringa oleifera, collected in different provinces in Thailand, were determined for the contents of total phenolics, total flavonoids, major components, and antioxidant activity. The extract and its major active components were investigated for the inhibition of H₂O₂-induced reactive oxygen species production and the effects on antioxidant enzymes mRNA expression. The extract, crypto-chlorogenic acid, isoquercetin and astragalin, significantly reduced the reactive oxygen species production inducing by H₂O₂ in HEK-293 cells. Treatment with isoquercetin significantly increased the mRNA expression levels of antioxidant enzymes such as superoxide dismutase, catalase and heme oxygenase 1. These results confirm that M. oleifera leaves are good sources of natural antioxidant with isoquercetin as an active compound.

Key words

Moringa oleifera - Moringaceae - antioxidant enzymes - isoquercetin - kidney cell - reactive oxygen species

^* Dedicated to Professor Dr. Dr. h. c. mult. Adolf Nahrstedt on the occasion of his 75th birthday.

• References

• 1 Turvey SE, Broide DH. Innate immunity. J Allergy Clin Immunol 2010; 125 (Suppl. 02) S24-S32
• 2 Jantan I, Bukhari SNA, Lajis NH, Abas F, Wai LK, Jasamai M. Effects of diarylpentanoid analogues of curcumin on chemiluminescence and chemotactic activities of phagocytes. J Pharm Pharmacol 2012; 64: 404-412
• 3 Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem 2009; 112: 303-309
• 4 Sunil C, Ignacimuthu S. In vitro and in vivo antioxidant activity of Symplocos cochinchinensis S. Moore leaves containing phenolic compounds. Food Chem Toxicol 2011; 49: 1604-1609
• 5 Pandanaboina SC, Kondeti SR, Rajbanshi SL, Kunala PN, Pandanaboina S, Pandanaboina MM, Wudayagiri R. Alterations in antioxidant enzyme activities and oxidative damage in alcoholic rat tissues: Protective role of Thespesia populnea . Food Chem 2012; 132: 150-159
• 6 Tanaka A, Hamada N, Fujita Y, Itoh T, Nozawa Y, Iinuma M, Ito M. A novel kavalactone derivative protects against H₂O₂-induced PC12 cell death via Nrf2/ARE activation. Bioorg Med Chem 2010; 18: 3133-3139
• 7 Hu W, Yu L, Wang MH. Antioxidant and antiproliferative properties of water extract from Mahonia bealei (Fort.) Carr. leaves. Food Chem Toxicol 2011; 49: 799-806
• 8 Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: a food plant with multiple medicinal uses. Phytother Res 2007; 21: 17-25
• 9 Pandey A, Pradheep K, Gupta R, Nayar ER, Bhandari DC. ‘Drumstick treeʼ (Moringa oleifera Lam.): a multipurpose potential species in India. Genet Resour Crop Evol 2010; 58: 453-460
• 10 Sahakitpichan P, Mahidol C, Disadee W, Ruchirawat S, Kanchanapoom T. Unusual glycosides of pyrrole alkaloid and 4′-hydroxyphenylethanamide from leaves of Moringa oleifera . Phytochemistry 2011; 72: 791-795
• 11 Chumark P, Khunawat P, Sanvarinda Y, Phornchirasilp S, Morales NP, Phivthong-Ngam L, Ratanachamnong P, Srisawat S, Pongrapeeporn KS. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J Ethnopharmacol 2008; 116: 439-446
• 12 Verma AR, Vijayakumar M, Rao CV, Mathela CS. In vitro and in vivo antioxidant properties and DNA damage protective activity of green fruit of Ficus glomerata . Food Chem Toxicol 2010; 48: 704-709
• 13 Iqbal S, Bhanger MI. Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J Food Compost Anal 2006; 19: 544-551
• 14 Vongsak B, Sithisarn P, Gritsanapan W. HPLC quantitative analysis of three major antioxidant components of Moringa oleifera leaf extracts. Planta Med 2012; 78: 1252
• 15 Fernandez J, Reyes R, Ponce H, Oropeza M, Vancalsteren MR, Jankowski C, Campos MG. Isoquercitrin from Argemone platyceras inhibits carbachol and leukotriene D4-induced contraction in guinea-pig airways. Eur J Pharmacol 2005; 522: 108-115
• 16 Ouédraogo M, Lamien-Sanou A, Ramdé N, Ouédraogo AS, Ouédraogo M, Zongo SP, Goumbri O, Duez P, Guissou PI. Protective effect of Moringa oleifera leaves against gentamicin-induced nephrotoxicity in rabbits. Exp Pathol 2013; 65: 335-339
• 17 Yeh CT, Ching LC, Yen GC. Inducing gene expression of cardiac antioxidant enzymes by dietary phenolic acids in rats. J Nutr Biochem 2009; 20: 163-771
• 18 Vongsak B, Sithisarn P, Gritsanapan W. Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions. Ind Crops Prod 2013; 49: 419-421
• 19 Faithong N, Benjakul S, Phatcharat S, Binsan W. Chemical composition and antioxidative activity of Thai traditional fermented shrimp and krill products. Food Chem 2010; 119: 133-140
• 20 Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem 2007; 100: 1511-1516
• 21 Kosem N, Han YH, Moongkarndi P. Antioxidant and cytoprotective activities of methanolic extract from Garcinia mangostana hulls. Sci Asia 2007; 33: 283-292
• 22 Hansen PB, Poulsen CB, Walter S, Marcussen N, Cribbs LL, Skott O, Jensen BL. Functional importance of L- and P/Q-type voltage-gated calcium channels in human renal vasculature. Hypertension 2011; 58: 464-470
• 23 Ferret PJ, Soum E, Negre O, Fradelizi D. Auto-protective redox buffering systems in stimulated macrophages. BMC Immunol 2002; 3: 3
• 24 Schmidt AJ, Hemmeter UM, Krieg JC, Vedder H, Heiser P. Impact of haloperidol and quetiapine on the expression of genes encoding antioxidant enzymes in human neuroblastoma SH-SY5Y cells. J Psychiatr Res 2009; 43: 818-823
• 25 Yoshiki N, Kubota T, Aso T. Identification of heme oxygenase in human endometrium. J Clin Endocrinol Metab 2001; 86: 5033-5038