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Horm Metab Res 2007; 39(4): 262-267
DOI: 10.1055/s-2007-973073
Original Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Difference of NPY and its Receptor Gene Expressions between Obesity and Obesity-resistant Rats in Response to High-fat Diet

C. Wang 1 , 2 , N. Yang 1 , S. Wu 1 , L. Liu 1 , X. Sun 1 , S. Nie 2
  • 1Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China
  • 2Department of Epidemiology and Biostatistic, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China
Further Information

Publication History

received 4. 9. 2006

accepted 29. 11. 2006

Publication Date:
19 April 2007 (online)

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Abstract

Aim: To investigate the effect of high-fat diet on expressions of hypothalamic neuropeptide Y (NPY) and Y1, Y2, Y5 receptors and the mechanism relative to the susceptibility of obesity.

Methods: Thirty-six female SD rats were randomly divided into high-fat diet group (HF, n=27) and chow food group (CF, n=9), and given either HF or CF diet for 13 weeks. Then the HF diet group was subdivided into dietary induced obesi-ty (DIO) and dietary induced obesity resistant (DIO-R) rat according to the final body weight. Body weight, caloric intake, energy efficiency, visceral fat pads were measured and compared. The levels of plasma and hypothalamic NPY were determined by Radioimmunoassay. Real-time Polymerase Chain Reaction (PCR) was used to measure the gene expression of NPY and its receptors in hypothalamus.

Results: Body weight, caloric intake, energy efficiency and visceral fat pads in DIO rats were higher significantly than those in CF or DIO-R rats (p<0.01). No difference in plasma NPY level was detected among the three groups (p>0.05); the hypothalamic NPY level was significantly higher in DIO group than in CF or DIO-R group (p<0.01). Gene expression levels of NPY and Y1, Y2, Y5 receptors were higher significantly in DIO group than in CF or DIO-R group (p<0.01), while no significant difference was found between DIO-R and CF rats (p>0.05), except that Y2 receptor was lower in DIO-R rats (p<0.01).

Conclusions: SD rat fed with a HF diet showed different susceptibility to obesity, and up-regulated hypothalamic NPY and Y1, Y2, Y5 receptor gene expressions were closely associated with being predisposed to obesity and overeating of DIO rats.

Key words

hypothalamus - neuropeptide Y - NPY receptor - obesity - caloric intake - energy efficiency - high-fat diet - dietary induced obesity

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Correspondence

Prof. N. Yang

Department of Nutrition and Food Hygiene

School of Public Health

Tongji Medical College

Huazhong University of Science and Technology

Hangkong Road 13

430030 Wuhan

PR China

Phone: +86/27/62434794

Fax: +86/27/83693763

Email: zynh@mails.tjmu.edu.cn

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