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Planta Med 1998; 64(5): 451-453
DOI: 10.1055/s-2006-957479
Papers
Phytochemical Analysis
© Georg Thieme Verlag Stuttgart · New York

Differentiation of Genuines and Counterfeits of Cordyceps Species using Random Amplified Polymorphic DNA

Kur-Ta Cheng1 , Ching-Hua Su2 , Hsien-Chang Chang1 , 3 , Jeng-Yi Huang4
  • 1Graduate Institute of Pharmacognosy Science, Taipei Medical College, Taipei, Taiwan, R. O. C.
  • 2Center of Biotechnology, Taipei Medical College, Taipei, Taiwan, R. O. C.
  • 3National Laboratories of Foods and Drugs Department of Health Executive Yuan, Taipei, Taiwan, R. O. C.
  • 4School of Pharmacy, Taipei Medical College, Taipei, Taiwan, R. O. C.
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Publikationsverlauf

1997

1998

Publikationsdatum:
04. Januar 2007 (online)

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Abstract

For identification of genuine and counterfeit samples of Cordyceps sinensis,.an RAPD (random amplified polymorphic DNA) analysis was performed. In the study, twelve samples were collected, of which six were genuine and six were counterfeit samples. Genuine samples of Cordyceps sinensis contain two parasitic larvae produced from each of the three provinces, Sichuan (SC), Chinghai (CH), and Tibet (TB). The counterfeits contain two products of each of the three species, Cordyceps hawkesii, Stachys geobombycis, and Stachys sieboldii. In the RAPD analysis, twenty arbitrary decamer primers were screened to obtain primers appropriate for differentiating the genuine and counterfeit samples. The result of twelve RAPD markers generated from four primers, OPT-08, OPT-12, OPT-13, and OPT-17, were selected. The primers can easily distinguish between genuine and counterfeit samples of Cordyceps sinensis.

Key words

Clavicipitaceae - Cordyceps sinensis - Cordyceps hawkesii - RAPD - Identification

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