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CC BY 4.0 · Rev Bras Ginecol Obstet 2018; 40(10): 625-630
DOI: 10.1055/s-0038-1670715
Review Article
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Effect of the Air Filtration System Replacement on Embryo Quality in the Assisted Reproduction Laboratory

Efeito da troca do sistema de filtragem do ar na qualidade embrionária no laboratório de reprodução assistida
Karine Queiroz Poletto
1   Faculty of Medicine, Centro Universitário UNIRG, Gurupi, TO, Brazil
,
Yanna Andressa Ramos de Lima
2   Center for Human Reproduction, Department of Gynecology and Obstetrics, Universidade Federal de Goiás, Goiânia, GO, Brazil
,
Mário Silva Approbato
2   Center for Human Reproduction, Department of Gynecology and Obstetrics, Universidade Federal de Goiás, Goiânia, GO, Brazil
› Author Affiliations
Further Information

Publication History

23 April 2018

03 July 2018

Publication Date:
23 October 2018 (online)

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Abstract

Improving infrastructural conditions of the in vitro fertilization laboratory, such as the air quality, has profound positive effects on embryo culture. Poor environmental conditions reduce the rate of embryo formation and, therefore, of pregnancy. This review article presents important publications regarding the impact of air quality in human reproduction laboratories on embryo quality, pregnancy success, and live births. The studies demonstrate that the replacing the air filtration system improves significantly the environmental air quality, and, consequently, improves laboratory parameters, such as the fertilization rate, the number of blastocysts, the embryo implantation rate, and the number of live births. On the other hand, improving air quality decreases the number of abortions. Therefore, environmental parameters that improve embryo quality and increase healthy child birth rates must be the main targets for the assisted reproduction laboratory quality control.

Resumo

Melhorar as condições de infraestrutura do laboratório de fertilização in vitro, com influência na qualidade do ar, tem efeitos positivos profundos na qualidade do embrião. As más condições ambientais do ar reduzem a taxa de sucesso na formação de embriões e a taxa de gravidez. Este artigo de revisão apresenta importantes publicações sobre o impacto da qualidade do ar dentro do laboratório de reprodução humana na qualidade do embrião, no sucesso de gravidez e no número de nascidos vivos. Os estudos demonstram que a troca do sistema de filtração de ar melhora significativamente a qualidade do ar ambiente, e consequentemente, melhora os parâmetros laboratoriais, tais como a taxa de fertilização, o número de blastocistos, a taxa de implantação e o número de nascidos vivos. Por outro lado, a melhora da qualidade do ar diminui o número de abortos. Portanto, os parâmetros ambientais que melhoram a qualidade do embrião e aumentam as taxas de nascimentos de crianças saudáveis devem ser os principais alvos para o controle de qualidade do laboratório de reprodução assistida.

Keywords

controlled environment - air quality - in vitro fertilization - embryo - volatile organic compounds

Palavras-chave

ambiente controlado - qualidade do ar - fertilização in vitro - embrião - compostos orgânicos voláteis
 

  • References

  • 1 Bhargava PM. On the critical assessment of the impact of the recent European Union Tissues and Cells Directive. Reprod Biomed Online 2005; 11 (02) 161 . Doi: 10.1016/S1472-6483(10)60953-4
    CrossrefPubMedGoogle Scholar
  • 2 Borges Jr E, Esteves S. Sala limpa Classe 100/ISSO 5: condição “Sine Qua Non” nos laboratórios de reprodução assistida? . Arq H Ellis. 2007; 3: 1-15
    PubMedGoogle Scholar
  • 3 Heitmann RJ, Hill MJ, James AN. , et al. Live births achieved via IVF are increased by improvements in air quality and laboratory environment. Reprod Biomed Online 2015; 31 (03) 364-371 . Doi: 10.1016/j.rbmo.2015.04.011
    CrossrefPubMedGoogle Scholar
  • 4 Esteves SC, Bento FC. Air quality control in the ART laboratory is a major determinant of IVF success. Asian J Androl 2016; 18 (04) 596-599 . Doi: 10.4103/1008-682X.166433
    CrossrefPubMedGoogle Scholar
  • 5 Esteves SC, Bento FC. Implementation of cleanroom technology in reproductive laboratories: the question is not why but how. Reprod Biomed Online 2016; 32 (01) 9-11 . Doi: 10.1016/j.rbmo.2015.09.014
    CrossrefPubMedGoogle Scholar
  • 6 Thomas T. Culture systems: air quality. Methods Mol Biol 2012; 912: 313-324 . Doi: 10.1007/978-1-61779-971-6_17
    CrossrefPubMedGoogle Scholar
  • 7 Albertini DF. Quality control in the ART laboratory: matters arising. J Assist Reprod Genet 2015; 32 (07) 1007-1008 . Doi: 10.1007/s10815-015-0539-6
    CrossrefPubMedGoogle Scholar
  • 8 European Union. Directive 2004/23/EC: On Setting Standards of Quality and Safety for the Donation, Procurement, Testing, Processing, Preservation, Storage and Distribution of Human Tissues and Cells . Brussels: EU; 2004. https://eur-lex.europa.eu/Lex-UriServ/LexUriServ.do?uri=OJ:L:2004:102:0048:0058:en:PDF . Accessed November 10, 2017
    PubMedGoogle Scholar
  • 9 Food and Drug Administration, HHS. Current good tissue practice for human cell, tissue, and cellular and tissue-based product establishments; inspection and enforcement. Final rule. Fed Regist 2004; 69 (226) 68611-68688
    PubMedGoogle Scholar
  • 10 Ministério da Saúde. Agência Nacional de Vigilância Sanitária. RDC/ANVISA N° 33, de 17 de fevereiro de 2006. Aprova o regulamento técnico para o funcionamento dos bancos de células e tecidos germinativos. Brasília, DF: Ministério da Saúde; 2006. http://adcon.rn.gov.br/ACERVO/Suvisa/doc/DOC000000000025025.PDF . Accessed September 10, 2017
    PubMedGoogle Scholar
  • 11 Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Resolução da Diretoria Colegiada - RDC/ANVISA N° 23, de 27 de maio de 2011. Dispõe sobre o regulamento técnico para o funcionamento dos Bancos de Células e Tecidos Germinativos e dá outras providências. Brasília, DF: Ministério da Saúde; 2011. http://portal.anvisa.gov.br/documents/10181/2954258/RDC_23_2011_COMP.pdf/ba335341-5993-4843-83dc-f23681690514 . Accessed November 10, 2017
    PubMedGoogle Scholar
  • 12 National Environmental Balancing Bureau. Procedural Standards for Certified Testing of Cleanrooms. 3rd ed. Gaithersburg, MD: NEBB; 2009. http://www.nebb.org/assets/1/7/PST_CR_20091.PDF . Accessed December 9, 2017
    PubMedGoogle Scholar
  • 13 Bernstein JA, Levin L, Crandall MS, Perez A, Lanphear B. A pilot study to investigate the effects of combined dehumidification and HEPA filtration on dew point and airborne mold spore counts in day care centers. Indoor Air 2005; 15 (06) 402-407 . Doi: 10.1111/j.1600-0668.2005.00379.x
    CrossrefPubMedGoogle Scholar
  • 14 Couto M. Monitoramento e controle microbiológico. Rev SBCC 2011; 55: 10-14
    PubMedGoogle Scholar
  • 15 Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Guia da Qualidade para Sistemas de Tratamento de Ar e Monitoramento Ambiental na Indústria Farmacêutica . Brasília, DF: ANVISA; 2013. http://conforlab.com.br/legislacao/qualidade_do_ar_final.pdf . Accessed February 5, 2018
    PubMedGoogle Scholar
  • 16 Boone WR, Johnson JE, Price TM. , et al. In vitro fertilization and embryo transfer (IVF-ET) comes to the upstate. J S C Med Assoc 1994; 90 (08) 360-366
    PubMedGoogle Scholar
  • 17 Boone WR, Johnson JE, Locke AJ, Crane 4th MM, Price TM. Control of air quality in an assisted reproductive technology laboratory. Fertil Steril 1999; 71 (01) 150-154 . Doi: 10.1016/S0015-0282(98)00395-1
    CrossrefPubMedGoogle Scholar
  • 18 Dickey RP, Wortham Jr JW, Potts A, Welch A. Effect of IVF laboratory air quality on pregnancy success. Fertil Steril 2010; 94: S151 . Doi: 10.1016/j.fertnstert.2010.07.605
    CrossrefPubMedGoogle Scholar
  • 19 Esteves SC, Gomes AP, Verza Jr S. Control of air pollution in assisted reproductive technology laboratory and adjacent areas improves embryo formation, cleavage and pregnancy rates and decreases abortion rate: comparison between a class 100 (ISO 5) and a class 1.000 (ISO 6) cleanroom for micromanipulation and embryo culture. Fertil Steril 2004; 82: S259-S260 . Doi: 10.1016/j.fertnstert.2004.07.691
    CrossrefPubMedGoogle Scholar
  • 20 Cohen J, Gilligan A, Esposito W, Schimmel T, Dale B. Ambient air and its potential effects on conception in vitro. Hum Reprod 1997; 12 (08) 1742-1749 . Doi: 10.1093/humrep/12.8.1742
    CrossrefPubMedGoogle Scholar
  • 21 Munch EM, Sparks AE, Van Voorhis BJ, Duran HE. Poor laboratory air quality and its impact on early embryo development. Fertil Steril 2014; 101: e14 . Doi: 10.1016/j.fertnstert.2013.11.075
    CrossrefPubMedGoogle Scholar
  • 22 Munch EM, Sparks AE, Duran HE, Van Voorhis BJ. Lack of carbon air filtration impacts early embryo development. J Assist Reprod Genet 2015; 32 (07) 1009-1017 . Doi: 10.1007/s10815-015-0495-1
    CrossrefPubMedGoogle Scholar
  • 23 Palter S, Dipaola K, Sparks AE. , et al. Multi-center study: innovative control of ambient air quality in multiple IVF laboratories is associated with statistically significant improvements in clinical outcomes - analysis of 5319 cycles. Fertil Steril 2016; 106: e27-e28 . Doi: 10.1016/j.fertnstert.2016.07.090
    CrossrefPubMedGoogle Scholar
  • 24 Hall J, Gilligan A, Schimmel T, Cecchi M, Cohen J. The origin, effects and control of air pollution in laboratories used for human embryo culture. Hum Reprod 1998; 13 (Suppl. 04) 146-155 . Doi: 10.1093/humrep/13.suppl_4.146
    CrossrefPubMedGoogle Scholar
  • 25 Legro RS, Sauer MV, Mottla GL. , et al. Effect of air quality on assisted human reproduction. Hum Reprod 2010; 25 (05) 1317-1324 . Doi: 10.1093/humrep/deq021
    CrossrefPubMedGoogle Scholar
  • 26 Checa Vizcaíno MA, González-Comadran M, Jacquemin B. Outdoor air pollution and human infertility: a systematic review. Fertil Steril 2016; 106 (04) 897-904.e1 . Doi: 10.1016/j.fertnstert.2016.07.1110
    CrossrefPubMedGoogle Scholar
  • 27 Higdon III HL, Graves JE, Blackhurst DW, Boone WR. Air quality within the incubator: will Volatile Organic Compound (VOC) filters make a difference in in vitro fertilization?. Fertil Steril 2003; 80: 260 . Doi: 10.1016/S0015-0282(03)01653-4
    CrossrefPubMedGoogle Scholar
  • 28 Morbeck DE. Air quality in the assisted reproduction laboratory: a mini-review. J Assist Reprod Genet 2015; 32 (07) 1019-1024 . Doi: 10.1007/s10815-015-0535-x
    CrossrefPubMedGoogle Scholar
  • 29 Choe S, Kim S, Jun Y. , et al. Ambient air pollution is associated with success of in-vitro fertilization cycles: a retrospective study in Seoul, Korea. Fertil Steril 2017; 108: e325 . Doi: 10.1016/j.fertnstert.2017.07.959
    CrossrefPubMedGoogle Scholar
  • 30 Worrilow KC, Huynh HT, Peters AJ. The innovative marriage between cleanroom and Assisted Reproductive Technologies (ART): the design, construction and National Environmental Balancing Bureau (NEBB) certification of a prototype class 100/class 10 IVF laboratory cleanroom. Fertil Steril 2000; 74: S103 . Doi: 10.1016/S0015-0282(00)01004-9
    CrossrefPubMedGoogle Scholar
  • 31 Johnson JE, Boone WR, Bernard RS. The effects of Volatile Compounds (VC) on the outcome of in vitro mouse embryo culture. Fertil Steril 1993; 60: S98-S99
    PubMedGoogle Scholar
  • 32 Esteves SC, Bento FC. Implementation of air quality control in reproductive laboratories in full compliance with the Brazilian Cells and Germinative Tissue Directive. Reprod Biomed Online 2013; 26 (01) 9-21 . Doi: 10.1016/j.rbmo.2012.10.010
    CrossrefPubMedGoogle Scholar
  • 33 Agarwal N, Chattopadhyay R, Ghosh S, Bhoumik A, Goswami SK, Chakravarty B. Volatile organic compounds and good laboratory practices in the in vitro fertilization laboratory: the important parameters for successful outcome in extended culture. J Assist Reprod Genet 2017; 34 (08) 999-1006 . Doi: 10.1007/s10815-017-0947-x
    CrossrefPubMedGoogle Scholar
  • 34 Carré J, Gatimel N, Moreau J, Parinaud J, Leandri R. Influence of air quality on the results of in vitro fertilization attempts: A retrospective study. Eur J Obstet Gynecol Reprod Biol 2017; 210: 116-122 . Doi: 10.1016/j.ejogrb.2016.12.012
    CrossrefPubMedGoogle Scholar
  • 35 Mahalingaiah S. Is there a common mechanism underlying air pollution exposures and reproductive outcomes noted in epidemiologic and in vitro fertilization lab-based studies?. Fertil Steril 2018; 109 (01) 68 . Doi: 10.1016/j.fertnstert.2017.10.034
    CrossrefPubMedGoogle Scholar
  • 36 Ha S, Sundaram R, Buck Louis GM. , et al. Ambient air pollution and the risk of pregnancy loss: a prospective cohort study. Fertil Steril 2018; 109 (01) 148-153 . Doi: 10.1016/j.fertnstert.2017.09.037
    CrossrefPubMedGoogle Scholar
  • 37 Cottell E, Lennon B, McMorrow J, Barry-Kinsella C, Harrison RF. Processing of semen in an antibiotic-rich culture medium to minimize microbial presence during in vitro fertilization. Fertil Steril 1997; 67 (01) 98-103 . Doi: 10.1016/S0015-0282(97)81863-8
    CrossrefPubMedGoogle Scholar
  • 38 Kastrop PM, de Graaf-Miltenburg LA, Gutknecht DR, Weima SM. Microbial contamination of embryo cultures in an ART laboratory: sources and management. Hum Reprod 2007; 22 (08) 2243-2248 . Doi: 10.1093/humrep/dem165
    CrossrefPubMedGoogle Scholar
  • 39 Ribeiro BRF, Amaral WN, Sadoyama G. Investigação bacteriológica e micológica de placas de cultivo de embriões em laboratório de reprodução humana. Reprod Clim. 2011; 26: 12-18
    PubMedGoogle Scholar
  • 40 Elder K, Baker D, Ribes J. Infections, Infertility and Assisted Reproduction . New York, NY: Cambridge University Press; 2005
    Google Scholar
  • 41 Foizer BRR, Silva KR, Vieira JDG, Amaral WN. Contaminação microbiológica em laboratório de reprodução humana e suas implicações no sucesso da reprodução assistida. Reprod Clim. 2014; 29: 66-70 . Doi: 10.1016/j.recli.2014.08.005
    PubMedGoogle Scholar
  • 42 Junqueira JRC, Alfieri AA. Falhas da reprodução na pecuária bovina de corte com ênfase para causas infecciosas. Semin Cienc Agrar 2006; 27: 289-298
    CrossrefPubMedGoogle Scholar
  • 43 Herlong JL, Reubish K, Higdon III HL, Boone WR. Quantitative and qualitative analysis of microorganisms in an assisted reproductive technology facility. Fertil Steril 2008; 89 (04) 847-853 . Doi: 10.1016/j.fertnstert.2007.04.019
    CrossrefPubMedGoogle Scholar
  • 44 Khoudja RY, Xu Y, Li T, Zhou C. Better IVF outcomes following improvements in laboratory air quality. J Assist Reprod Genet 2013; 30 (01) 69-76 . Doi: 10.1007/s10815-012-9900-1
    CrossrefPubMedGoogle Scholar
  • 45 Kresowik J, Duran HE, Sparks A, Van Voorhis B. The impact of suboptimal air quality in embryology laboratory on IVF outcome. Fertil Steril 2012; 98: S285-S286 . Doi: 10.1016/j.fertnstert.2012.07.1037
    CrossrefPubMedGoogle Scholar