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Planta Med 2023; 89(02): 218-230
DOI: 10.1055/a-1942-5428
Natural Product Chemistry & Analytical Studies
Original Papers

UPLC/Q-TOF-MS-based Metabolomics Study of the Antiosteoporosis Effects of Vaccarin in Ovariectomized Mice

Fangming Song   *
1   Research Centre of Regenerative Medicine, Guangxi Medical University, Nanning City, China
,
Tianyu Xie   *
2   Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China
,
Xi Liu   *
3   College of Chemistry and Chemical Engineering, Xiamen University, Nanning City, China
,
Bonnie Chin
4   School of Postgraduate Studies, International Medical University, Kuala Lumpur, Malaysia
,
Xiaoting Luo
5   Department of Pharmacy, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China
,
Shijie Liao
1   Research Centre of Regenerative Medicine, Guangxi Medical University, Nanning City, China
,
Abu·Moro,
Wenyu Feng
2   Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China
,
Mingwei He
2   Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China
,
Nenggan Huang
2   Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China
,
Zhiheng Su
7   Pharmaceutical College, Guangxi Medical University, Nanning City, China
,
Yun Liu
6   Department of Spine and Bone Diseases, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China
1   Research Centre of Regenerative Medicine, Guangxi Medical University, Nanning City, China
› InstitutsangabenGefördert durch: Natural Science Foundation of Guangxi Province 2017GXNSFBA198061
Gefördert durch: Natural Science Foundation of Guangxi Province 2019JJA140408
Gefördert durch: Youth Science and Technology Venus Project of the First Affiliated Hospital of Guangxi Medical University 201903038
Gefördert durch: National Natural Science Foundation of China 81960768
Gefördert durch: Project funded by China Postdoctoral Science Foundation 2018M633618XB
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Abstract

Osteoporosis is a systemic and metabolic bone disease that usually occurs in postmenopausal women, which mainly manifests as bone loss and increased bone fragility that both facilitate fracture. However, few drugs for osteoporosis have shown good efficacy and limited side effects. Vaccarin has demonstrated its antiosteoporosis effects by inhibiting the formation and osteolytic activities of osteoclasts in our previous investigation. In this study, multivariate statistical analysis and ultrahigh-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry were used to analyze the serum metabolites of ovariectomized mice treated with or without vaccarin. As a result, 9 serum metabolites were identified as biomarkers. The metabolic levels of 3 crucial biomarkers, namely, lysophosphatidylcholine [22 : 6, (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)], 1-linoleoylglycerophosphocholine and 1-palmitoyl-Sn-glycero-3-phosphocholine, that were correlated with glycerophospholipid metabolism increased and then decreased significantly after vaccarin treatment. Molecular docking analysis and osteoclasts differentiation experiment further revealed that vaccarin may bind with phospholipase A2 and downregulated its activity to reduce the osteoclastogenesis. Therefore, the occurrence of osteoporosis is closely related with glycerophospholipid metabolism disorders, and vaccarin exerts antiosteoporosis effects by reducing the levels of glycerophospholipid metabolites.

Key words

postmenopausal osteoporosis - glycerophospholipids metabolism - phospholipase A2 - vaccarin - Vaccaria segetalis - Caryophyllaceae

* These authors have contributed equally to this work and share first authorship.


Supporting Information

    The overlapped ion chromatograms of all QC samples, the MS spectra of tested sample, the supplementary figures and the supplementary tables are available in Supporting Information.

  • Ergänzendes Material


Publikationsverlauf

Eingereicht: 10. Februar 2022

Angenommen nach Revision: 13. September 2022

Accepted Manuscript online:
13. September 2022

Artikel online veröffentlicht:
10. Januar 2023

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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