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Planta Med 2023; 89(11): 1052-1062
DOI: 10.1055/a-1728-2249
Biological and Pharmacological Activity
Original Papers

Microbiological and Clinical Effects of a Proanthocyanidin-enriched Extract from Rumex acetosa in Periodontally Healthy Carriers of Porphyromonas gingivalis: a Randomized Controlled Pilot Study

Sabine Selbach 
1   Adelaide Dental School, The University of Adelaide, Adelaide, Australia
,
Astrid Klocke
2   Private Practice, Krefeld, Germany
,
Ulrike Peters
3   Department of Periodontics, Preventive and Restorative Dentistry, University Medical Center Eppendorf, Hamburg, Germany
,
Sabine Beckert
4   Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
,
Rory Munro Watt
5   Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Peoplesʼ Republic of China
,
Raymond Tong
5   Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Peoplesʼ Republic of China
,
Thomas Frank Flemmig
5   Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, Peoplesʼ Republic of China
,
Andreas Hensel 
4   Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
,
Thomas Beikler
3   Department of Periodontics, Preventive and Restorative Dentistry, University Medical Center Eppendorf, Hamburg, Germany
› InstitutsangabenGefördert durch: European Union (ZIEL.2.NRW program) 300262502 to TB
Gefördert durch: European Union (ZIEL.2.NRW program) w1006sb014 to AH
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Abstract

Rumex acetosa significantly inhibits the adhesion of Porphyromonas gingivalis (P. g.) to eukaryotic host cells in vitro. The objective of this randomized placebo-controlled pilot-trial was to analyze effects of a mouth rinse containing 0.8% (w/w) of a quantified proanthocyanidin-enriched extract from Rumex acetosa (RA1) on microbiological, clinical, and cytological parameters in systemically healthy individuals without history of periodontitis, harboring P. g. intraorally. 35 subjects received a supragingival debridement (SD) followed by mouth rinsing (3 times daily) with either RA1 mouth rinse solution (test) or placebo (control) for 7 days as adjunct to routine oral hygiene. Supragingival biofilm samples were taken at screening visit, baseline (BL), 2, 4, 7 and 14 days after SD. P. g. and 11 other oral microorganisms were detected and quantified by rtPCR. Changes in the oral microbiota composition of one test and one control subject were assessed via high throughput 16S rRNS gene amplicon sequencing. Approximal Plaque Index (API) and the modified Sulcular Bleeding Index (SBI) were assessed at BL, 7- and 14-days following SD. Brush biopsies were taken at BL and 14 d following SD. Intergroup comparisons revealed no significant microbiological, cytological, and clinical differences at any timepoint. However, a significant reduction in SBI at day 14 (p = 0.003) and API at day 7 (p = 0.02) and day 14 (p = 0.009) was found in the test group by intragroup comparison. No severe adverse events were observed. The results indicate that RA1 mouth rinse is safe but does not seem to inhibit colonization of P. g. or improve periodontal health following SD.

Key words

Rumex acetosa - Polygonaceae - proanthocyanidines - anti-adhesive - Porphyromonas gingivalis

Supporting Information

    The references for the bacteria specific primers used in the qrtPCR (see Table 1S) are listed in the Supporting Information.

    The results of high throughput 16S rRNA gene sequence analysis of the oral bacterial microbiome from one control (SA120) and one test subject (SA088), at each time-point (V1 – V6), are presented in the Supporting Information (see Fig. 1S, 2S).

  • Supporting Information


Publikationsverlauf

Eingereicht: 24. August 2021

Angenommen nach Revision: 25. Dezember 2021

Accepted Manuscript online:
25. Dezember 2021

Artikel online veröffentlicht:
28. Februar 2022

© 2022. Thieme. All rights reserved.

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Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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